Purification, Isolation, and Characterization of Esterase from Rhodococcus sp. LKE-021
نویسندگان
چکیده
منابع مشابه
Extracellular Cholesterol Oxidase from Rhodococcus sp.: Isolation and Molecular Characterization
Background: Cholesterol oxidase (CHO) has various clinical and industrial applications. Recently, microbial CHO have received a great attention for their wide usage in medicine. Here, taxonomic characterizations of isolated strain from soil, optimization of the conditions for CHO production and biochemical characterizations of produced CHO enzyme were described. Finally, CHO gene was cloned int...
متن کاملextracellular cholesterol oxidase from rhodococcus sp.: isolation and molecular characterization
background: cholesterol oxidase (cho) has various clinical and industrial applications. recently, microbial cho have received a great attention for their wide usage in medicine. here, taxonomic characterizations of isolated strain from soil, optimization of the conditions for cho production and biochemical characterizations of produced cho enzyme were described. finally, cho gene was cloned int...
متن کاملExtracellular cholesterol oxidase from Rhodococcus sp.: isolation and molecular characterization.
BACKGROUND Cholesterol oxidase (CHO) has various clinical and industrial applications. Recently, microbial CHO have received a great attention for their wide usage in medicine. Here, taxonomic characterizations of isolated strain from soil, optimization of the conditions for CHO production and biochemical characterizations of produced CHO enzyme were described. Finally, CHO gene was cloned into...
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Laccases are blue copper oxidases (E.C. 1.10.3.2 benzenediol: oxygen oxidoreductase) that catalyze the one-electron oxidation of phenolics, aromatic amines, and other electron-rich substrates with the concomitant reduction of O(2) to H(2)O. They are currently seen as highly interesting industrial enzymes because of their broad substrate specificity. A positive strain was isolated and characteri...
متن کاملExpression, purification and kinetic characterization of recombinant benzoate dioxygenase from Rhodococcus ruber UKMP-5M
In this study, benzoate dioxygenase from Rhodococcus ruber UKMP-5M was catalyzed by oxidating the benzene ring to catechol and other derivatives. The benzoate dioxygenase (benA gene) from Rhodococcus ruber UKMP-5M was then expressed, purified, characterized, The benA gene was amplified (642 bp), and the product was cloned into a pGEM-T vector.The recombinant plasmid pGEMT-benA was digested by d...
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ژورنال
عنوان ژورنال: Journal of Pure and Applied Microbiology
سال: 2020
ISSN: 0973-7510,2581-690X
DOI: 10.22207/jpam.14.2.36